The result from the GM6001 metalloproteinase inhibitor around the regeneration of ambulacral structures in Eupentacta fraudatrix continues to be investigated. Inhibition of proteinase activity exerts reasonable impact on regeneration, being determined by time when GM6001 is injected. When administration from the inhibitor begins on day 3 publish-injuries, regeneration is totally abolished, and also the creatures die. Which means that early activation of proteinases is vital for triggering the regenerative process in holothurians. When GM6001 in first injected on day 7 publish-injuries, the regeneration rate decreases. However, this effect has shown to be reversible: when inhibition ceases, the regeneration resumes. The result from the inhibitor is manifested like a retarded degradation from the extracellular matrix, the possible lack of cell dedifferentiation, and, most likely, a slower cell migration. The gelatinase activity is detected out of all regenerating organs of E. fraudatrix. Within the holothurian Cucumaria japonica, which isn’t able to healing skin wounds and ambulacrum reparation, no gelatinase activity was observed to begin of harm. An indicator is created that proteinases play a huge role in regeneration in holothurians. Probably the most probable morphogenesis regulators are matrix metalloproteinases with gelatinase activity.